BCL-2, His-Tag Recombinant

Catalog #

50272

$450 *

Size: 100 µg

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Description

Recombinant human BCL-2 (apoptosis regulator Bcl-2), encompassing amino acids 1-211. This construct contains a C- terminal His-tag (10xHis). The recombinant protein was affinity purified.

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Synonyms

Bcl, Bcl2, B cell lymphoma 2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BCL-2 Activity

BCL-2 inhibition curve using a TR-FRET assay (#50222-2). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

BCL-2 (1-211-His)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and variable imidazole

25 kDa

Amino Acids

1–211

Genbank #

NM_000633

UniProt #

P10415

Tag(s)

C-terminal His-tag

Background

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

References

1. Labi V. et al., Cell Death Differ. 2008 Jun;15(6):977-87.
2. Rong Y.P. et al., Mol Cell. 2008 Jul 25;31(2):255-65.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to BCL-2 TR-FRET Assay Kit (BPS Bioscience #50222) with BCL-2 titrated from 12 ng/rxn-0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Apoptosis

Regulatory Status

Research Use Only

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Datasheets/Protocols

50272 Lot 191001-G

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