BCL2A1 TR-FRET Assay Kit
The BCL2A1 TR-FRET Assay Kit is designed to measure the inhibition of BCL2A1 (BFL1) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2A1, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against BCL2A1? Check out our Apoptosis Screening Services.
Dithiothreitol (DTT, 0.5 M)
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
| Catalog Number | Component | Amount | Storage | |
| 100077 | BCL2A1 | 20 µg | 80°C |
|
| BCL2 Peptide Ligand 2 | 400 rxns | 80°C | ||
| 30017 | Anti-His Tb-labeled donor | 2 x 10 µl | -20°C | |
| Dye-labeled acceptor | 2 x 10 µl | -20°C | ||
| 3x MCL TR-FRET Assay Buffer* | 4 ml | -20°C | ||
| White, nonbinding, low volume, microtiter plate | 1 | Room Temp. | ||
* Add 30 µl of 0.5M DTT before use
Kvansakul, M., Hinds, MG. Cell Death and Disease 2013; 4: e909.
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