MCL-1 TR-FRET Assay Kit (Mouse)

Catalog #
79928
$870 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The Mouse MCL-1 TR-FRET Assay Kit is designed to measure the inhibition of mouse MCL-1 binding to its ligand in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; TR-FRET signal can be measured after 1-hour incubation of a sample containing terbium (Tb)-labeled donor, dye-labeled acceptor, recombinant Mouse MCL-1 protein, peptide ligand, and an inhibitor.

Need us to run inhibitor screens or profile your compounds against MCL-1(Mouse)? Check out our Apoptosis Screening Services or DNA Replication and Repair Screening Services.​

Synonyms
MCL1 assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
TR-FRET
Supplied As
384 reaction format.
Materials Required But Not Supplied

Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format
Catalog # Component Amount Storage
100637 Mouse MCL-1 10 μg -80°C

Avoid
freeze/
thaw
cycles

79507 MCL-1 Peptide Ligand 400 rxns -80°C
30017 Anti-His Tb-labeled donor 2 x 10 μl -20°C
  Dye-labeled acceptor 2 x 10 μl -20°C
  3x MCL TR-FRET Assay Buffer* 4 ml -20°C
79969 White, nonbinding, low volume, microtiter plate 1 Room
Temp
 
UniProt #
P97287
References

Abid, M., et al. Curr Med Chem, 2017; 24:4488-4514.