MCL-1 TR-FRET Assay Kit (Mouse)
The Mouse MCL-1 TR-FRET Assay Kit is designed to measure the inhibition of mouse MCL-1 binding to its ligand in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; TR-FRET signal can be measured after 1-hour incubation of a sample containing terbium (Tb)-labeled donor, dye-labeled acceptor, recombinant Mouse MCL-1 protein, peptide ligand, and an inhibitor.
Need us to run inhibitor screens or profile your compounds against MCL-1(Mouse)? Check out our Apoptosis Screening Services or DNA Replication and Repair Screening Services.
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Catalog # | Component | Amount | Storage | |
100637 | Mouse MCL-1 | 10 μg | -80°C |
Avoid |
79507 | MCL-1 Peptide Ligand | 400 rxns | -80°C | |
30017 | Anti-His Tb-labeled donor | 2 x 10 μl | -20°C | |
Dye-labeled acceptor | 2 x 10 μl | -20°C | ||
3x MCL TR-FRET Assay Buffer* | 4 ml | -20°C | ||
79969 | White, nonbinding, low volume, microtiter plate | 1 | Room Temp |
Abid, M., et al. Curr Med Chem, 2017; 24:4488-4514.