MCL-1 TR-FRET Assay Kit
The MCL-1 TR-FRET Assay Kit is designed to measure the inhibition of MCL-1 binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, MCL-1, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against MCL-1? Check out our Apoptosis Screening Services or DNA Replication and Repair Screening Services.
This product has been cited 4 times.
Catalog # | Component | Amount | Storage | |
40742 | MCL-1 | 10 µg | -80°C | Avoid freeze/ thaw cycles! |
79507 | MCL-1 Peptide Ligand | 400 rxns | -80°C | |
30017 | Anti-His Tb-labeled donor | 10 µl | -20°C | |
Dye-labeled acceptor | 10 µl | -20°C | ||
3x MCL TR-FRET Assay Buffer | 4 ml | -20°C | ||
79969 | White, nonbinding, low volume, microtiter plate | 1 | Room Temp. |
1. Abid, M., et al. Curr Med Chem 2017; 24:4488