IRAK4 Kinase Assay Kit
The IRAK4 Kinase Assay Kit is designed to measure IRAK4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
- ADP-Glo® Kinase Assay (Promega #6930)
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40064 | IRAK4, GST-tag Recombinant* | 2.5 µg | -80°C |
| 79334 | Kinase assay buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 100 µl | -20°C |
| 78514 | Myelin basic protein (MBP), 5 mg/ml | 100 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
IRAKs (interleukin receptor associated kinases) are a family of Serine/Threonine kinases that induce a protective acute inflammation response, as well as an adaptive immune response in the presence of pathogens. They are associated with Toll-like receptors in immune cells and transduce an intracellular signaling cascade that results in the transcription of genes involved in inflammation. Four IRAK-like molecules have been identified IRAK1, IRAK2, IRAK4 and IRAKM. IRAK1 and IRAK4 are active kinases, while IRAK2 and IRAKM are inactive. All four kinases mediate activation of NFκB (nuclear factor kappa B), a regulator of innate immunity through signaling pathways that link pathogenic and cellular danger signals in response to invading pathogens. IRAKs have the potential to be used as therapeutic targets due to their highly functional role as mediators of immune response.
Janssens, S., et al,. Molecular Cell, (2003). 11(2), 293–302.
Powered by Bioz
San Diego, CA 92121
858-202-1401
Products are for Research Use Only
COMPANY
Copyright © 2024
BPS Bioscience, Inc.
All rights reserved.
Diagrams on this website are created with BioRender.com.