IRAK1, His-Tag Recombinant

Catalog #

40202

$365 *

Size: 10 μg

Qty

Bulk/Custom

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Description

Recombinant human IRAK1 (interleukin-1 receptor-associated kinase 1), encompassing amino acids 186-530. This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

Interleukin-1 receptor-associated kinase 1, IRAK-1, IRAK

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

IRAK1 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

IRAK1 (His-186-530)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, variable imidazole, and 20% glycerol.

40 kDa

Amino Acids

186-530

Specific Activity

≥40 pmol/min/µg

Genbank #

NM_001569

UniProt #

P51617

Tag(s)

N-terminal His-tag

References

1. Ku C.L. et al., J. Exp. Med. 2007; 204(10): 2407-2422.

2. Hoarau C. et al., J. Immunol. 2007; 179(7): 4754-4765.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40202 Lot 180331-1

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IRAK1, His-Tag Recombinant

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