GSK3α Recombinant

Catalog #

40006

$365 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human GSK3α, also known as GSK3a, glycogen synthase kinase 3 alpha, GSK3alpha, GenBank Accession No. BC027984, a.a. 2-483(end), with an N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=78 kDa.

This product has been cited 5 times.

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Synonyms

GSK3a, glycogen synthase kinase 3 alpha, GSK3alpha

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations5

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥81%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM GSH, 3 mM DTT, 20% glycerol

81.5 kDa

Amino Acids

2-483(end)

Specific Activity

≥ 500 pmol/min/ug.

Genbank #

BC027984

UniProt #

P49840

Tag(s)

N-terminal GST-tag

References

1. Meijer, L. et al., Trends Pharmacol Sci. 25 (9), 471-480 (2004).
2. Patel, S. et al., Biochem Soc. Trans. 32(pt5): 803-808 (2004).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Enzyme reactions include 50 μL volumes of 1x Kinase buffer (40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA) along with 20 μM ATP, 0.2 mg/mL GSKtide substrate, 3mM DTT, and GST-GSK3α. After 45 min incubation at 30°C, 50μL of the Kinase-Glo reagent (Promega) solution was added and luminescence from remaining ATP was measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40006 Lot 130613-H1.pdf

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