ERK1 Kinase Assay Kit

Catalog #

82282

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ ERK1 Kinase Assay Kit is designed to measure ERK1 (extracellular signal-regulated kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ERK1, MBP, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1 (ERK-1), Insulin-stimulated MAP2 kinase, MAP kinase isoform p44 (p44-MAPK), Microtubule-associated protein 2 kinase, p44-ERK1

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Product Data Gallery

Inhibition of ERK1 kinase activity by Pluripotin

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	ERK1	1.25 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
78514	MBP (5 mg/ml)	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

P27361

Background

ERK1 (extracellular signal-regulated kinase 1) is also known as mitogen-activated protein kinase 3 (MAPK3), is a dual serine/threonine and tyrosine kinase. ERK (extracellular signal-regulated kinases), also known as classical MAPK (mitogen-activated protein kinases), are protein kinases involved in crucial intracellular signaling pathways, and regulate processes like mitoses, meiosis, and other functions. In the MAPK/ERK pathway Ras proteins are activated by several external molecules, including cytokines and viruses. These are followed by an activation cascade of c-Raf, MEK (mitogen-activated protein kinase kinase) and finally ERK. ERK1/2 in turn activates transcription factors. Disruption of the MAPK/ERK pathway at any point results in cancer. Inhibitors for Ras, Raf and MEK have been developed and result in rapid tumor reductions. However, resistance to treatment and relapse are quite frequent. This has led the field to focus on the development of ERK inhibitors, although these are difficult to design due to the high similarity to the CDK (cyclin-dependent kinase) pockets. Several ERK1/2 inhibitors are undergoing clinical trials aiming at treating advanced solid tumors, such as melanoma and adenocarcinoma, but further development and studies into ERK2 will open new therapeutic strategies in cancer oncology.

References

Busca R. et al., 2016 Front Cell Dev Biol 4:53.
Lavoie H., et al., 2020 Nature Reviews Molecular Cell Biology 21:607-623

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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ERK1 Kinase Assay Kit

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