Chemi-Verse™ CLK3 Kinase Assay Kit

Catalog #

82150

$520 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ CLK3 Kinase Assay Kit is designed to measure CLK3 (CDC-like kinase 3) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CLK3 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

PHCLK3, FLJ22858, PHCLK3/152

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Product Data Gallery

Inhibition of CLK3 kinase activity by Staurosporine.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40198	CLK3, GST-Tag Recombinant*	2.5 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
78514	Myelin basic protein (MBP), 5 mg/ml	50 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

P49761

Background

CDC-like kinase 3 (CLK3) is part of the dual-specificity protein kinase (DSK) family that phosphorylate serine/arginine rich (SR) proteins that are part of the spliceosomal complex and also regulate non-splicing proteins. Dysfunction in pathways regulated by CLK3 can result in cancer. CLK3 is upregulated in cholangiocarcinoma (CCA), hepatocellular carcinoma (HCC) and hematopoietic stem cells pathologies. The mechanism of action in each disease seems to be specific, with CLK3 activating Wnt3a transcription and increasing cell proliferation and migration in HCC, but it works in a CLK3-USP13-c-Myc feedback loop in CCA. CLK3 also contributes to HIV infection by promoting the expression of Gag and viral RNA. The use of chlorhexidine, a CLK2 /3/4 inhibitor, for HIV-1 treatment has shown in promising results in decreasing viral replication and prevention of mother-to-child transmission (MTCT). The development of CLK3 specific inhibitors and a deeper understanding of its role in the multiple pathways where it is involved will prove crucial for the treatment of CLK3-linked diseases.

References

Song M., et al., 2023 Signal Transduction and Targeted Therapy 8: 148.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ CLK3 Kinase Assay Kit

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