Chemi-Verse™ CLK1 Kinase Assay Kit

Catalog #

82145

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ CLK1 Kinase Assay Kit is designed to measure CLK1 (CDC2-like kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CLK1 kinase (amino acids 129-end), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

CLK, STY, CLK/STY

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Product Data Gallery

Inhibition of CLK1 kinase activity by Staurosporine.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40196	CLK1, GST-Tag*	2.5 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
78514	Myelin Basic Protein (MBP), 5 mg/ml	50 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

P49759

Background

CDC-like kinase 1 (CLK1) is part of the dual-specificity protein kinase (DSK) family that phosphorylateserine/ arginine rich (SR) proteins that are part of the spliceosomal complex and regulate non-splicing proteins. It is involved in cell cycle progression, apoptosis, the DNA replication checkpoint, and regulation of telomere length. Dysfunctions in pathways regulated by CLK1 result in Alzheimer’s disease, Duchenne’s muscular dystrophy (DMD), viral replication, autophagy-associated diseases (diabetes, cardiovascular, and infectious diseases) and cancer. CLK1 is upregulated in prostate cancer, gastric, and pancreatic ductal adenocarcinoma. The use of TG003, a CLK1/2/4 inhibitor, leads to dystrophin exon31 skipping in cells from DMD patients, and a partially functional dystrophin protein. TG003 also decreases gastric and prostate cancer cell viability and migration. CLK1 inhibitors such as NIH39 and KH-CB19 showed anti-viral activity towards influenza A, and Leucettine L41 increases cellular autophagy in U-2 OS cells. The development of CLK1 inhibitors and a deeper understanding of its role in the multiple pathways where it is involved will prove crucial for the treatment of CLK1-linked diseases.

References

Song M., et al., 2023 Signal Transduction and Targeted Therapy 8: 148.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ CLK1 Kinase Assay Kit

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