MUC16 (CA125), variant 4 (region 13785-14507) CHO Cell Line

Catalog #

78848

$7,150 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Engineered CHO cell line stably expressing human C-terminus MUC16 (CA125) transcript variant 4, from amino acid 13785 to 14507 (NM_024690.2). This portion of MUC16 contains several of the extracellular SEA modules, the transmembrane domain, and the short cytoplasmic tail of the protein. Surface expression of MUC16 was confirmed by flow cytometry. This stable clonal cell line was selected based on its high level of MUC16 expression compared to the parental CHO-K1 cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Flow cytometry analysis of MUC16 expression in MUC16 (CA125), variant 4 (region 13785-14507) CHO Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3J	BPS Bioscience #79974

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

MUC16 (also known as Mucin16, Cell Surface Associated, and ovarian cancer marker CA125) is a protein of the mucin family. Mucins are high molecular weight, O-glycosylated proteins found on the apical surfaces of epithelia. MUC16 has been found in the cornea, the respiratory system, and the female reproductive system, where it plays a role in protecting epithelial cells from pathogen entry. MUC16 dysfunction has been associated with dry eye, cystic fibrosis and cancer, in particular ovarian cancer. MUC16 binds to mesothelin and may be involved in migration and metastasis of tumor cells. Currently MUC16 is used as biomarker for certain cancer types, in addition to being studied in the context of cell signaling. Due to its high expression in some tumors, it is investigated as a therapeutic target for antibody-based therapy as well as adaptive cell therapy.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3J (BPS Bioscience #79974): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin, plus 5 µg/ml of Puromycin.

Applications

Screen and validate antibodies against MUC16 for drug discovery and research.
Perform binding assays to screen for potential MUC16 ligands.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For further questions, email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only