Cereblon/DDB1 Complex Recombinant

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Catalog #

102166

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Description

Complex of recombinant full length human Cereblon, amino acids 1-442(end), and full length DDB1 (damage specific DNA binding protein 1), amino acids 1-1140(end). The cereblon construct includes an N-terminal FLAG-tag, while the DDB1 construct contains a C-terminal Strep-tag. The proteins were co-expressed and purified as a complex.

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Synonyms

Protein cereblon, AD-006, CRBN, DDB p127 subunit, DNA damage-binding protein a (DDBa), Damage-specific DNA-binding protein 1, HBV X-associated protein 1 (XAP-1), UV-damaged DNA-binding factor

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Construct

Cereblon (FLAG-1-442(end)) / DDB1 (1-1140(end)-Strep)

Host Species/Expression System

HEK293

Purity

≥75%

Format

Aqueous buffer solution.

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glycerol

Cereblon: 53 kDa; DDB1: 131 kDa

Amino Acids

Cereblon: 1-442(end); DDB1: 1-1140(end)

Genbank #

Cereblon: NM_016302; DDB1: NM_001923

UniProt #

CRBN: Q96SW2; DDB1: Q16531

Tag(s)

N-Terminal FLAG-Tag, C-Terminal Strep-Tag

Background

Cereblon (CRBN) is the substrate-binding component of the E3 protein ligase complex DDB1-CUL4A-RBX1, which is involved in the ubiquitination and proteasomal degradation of target proteins. Binding of CRBN to a substrate engages its E3 ligase activity and results in the ubiquitination and ultimate degradation of the substrate. Many proteins are known targets of CRBN, including several transcription factors, growth factors and kinases. CRBN has become a target of choice for the development of many PROTACs of therapeutical interest.

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Datasheets/Protocols

102166 Lot 240311-2

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Cereblon/DDB1 Complex Recombinant

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