BRD4 (BD1+BD2) Recombinant

Catalog #

31047

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Bromodomain containing 4 (also known as BRD4, HUNK1, or MCAP), encompassing amino acids 49-460 corresponding to bromodomains 1 and 2. This construct does not contain a tag.

This product has been cited 15 times.

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Synonyms

Bromodomain-containing protein 4, Protein HUNK1, MCAP, BRD-4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BRD4 (BD1+BD2) Activity

Product Info

Storage and Usage

Citations15

Species

Human

Construct

BRD4 (BD1+BD2) (49-460)

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.

47 kDa

Amino Acids

49–460

Genbank #

NM_058243

UniProt #

O60885

References

1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May; 90(5): 587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov 29;12(11):133.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was performed according to BRD4 (BD1+BD2) TR-FRET Assay Kit (BPS Bioscience #32612) with BRD4 (BD1+BD2) Recombinant titrated at various concentrations as a competitive antagonist to BRD4 (BD1+BD2) GST-Tag Recombinant (BPS Bioscience #31044).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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Datasheets/Protocols

31047 Lot 170427-4

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BRD4 (BD1+BD2) Recombinant

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