BRD4 (BD1+BD2), GST-Tag Recombinant

Catalog #

31044

$315 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human BRD4 (bromodomain containing 4), encompassing amino acids 79-460. This construct contains an N-terminal GST-tag followed by a PreScission Protease Cleavage Site for removal of GST-tag. The recombinant protein was affinity purified.

This product has been cited 6 times.

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Synonyms

Bromodomain containing 4, BRD4, HUNK1, MCAP, BRD-4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BRD4 (BD1+BD2) Activity

BRD4 (BD1+BD2) inhibition curve using a TR-FRET assay (#32612). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations6

Species

Human

Construct

BRD4 (BD1+BD2) (GST-49-460)

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol

73 kDa

Amino Acids

49-460

Genbank #

NM_058243

UniProt #

O60885

Tag(s)

N-terminal GST-tag

References

Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to BRD4 (BD1+BD2) TR-FRET Assay Kit (BPS Bioscience #32612) with BRD4 (BD1+BD2) (BPS Bioscience #31044) titrated from 72 ng/rxn – 1.125 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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Datasheets/Protocols

31044 Lot 201111-3

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BRD4 (BD1+BD2), GST-Tag Recombinant

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