BRD4 (BD1+BD2), His-Tag Recombinant

Catalog #

31045

$315 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human BRD4 (Bromodomain-containing protein 4, also known as HUNK1), encompassing amino acids 49-460 corresponding to bromodomains 1 and 2. This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

This product has been cited 1 time.

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Synonyms

Bromodomain containing protein 4, BRD4, HUNK1, MCAP, BRD-4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BRD4 (BD1 + BD2) Activity

Product Info

Storage and Usage

Citations1

Species

Human

Construct

BRD4 (BD1+BD2) (His-49-460)

Host Species/Expression System

E. coli

Purity

≥85%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glycerol.

47 kDa

Amino Acids

49-460

Genbank #

NM_058243

UniProt #

O60885

Tag(s)

N-terminal His-tag

References

1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov 29;12(11):133.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

BRD4 (BD1+BD2), His-tag (BPS Bioscience #31045) protein is incubated with biotinylated acetyl-histone peptide substrate at 50 nM (BPS Bioscience #33000) in 3x BRD Homogeneous Assay Buffer 1 (BPS Bioscience #33001) in a 10 µl reaction for 1 hour at RT. 20 µl of 1:1 mixture AlphaLISA^® Nickle Chelate acceptor beads (PerkinElmer AL108C) diluted 1:250 fold and Alphascreen^® Streptavidin -conjugated donor beads (PerkinElmer 6760002S) diluted 1:125 fold was added and incubated for 1 hour at room temperature. After 1 hour incubation at RT, Alphacounts are measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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Datasheets/Protocols

31045 Lot 220302

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BRD4 (BD1+BD2), His-Tag Recombinant

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