PRDM9, GST-Tag Recombinant

Catalog #

100078

$440 *

Size: 50 μg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human PRDM9, also known as PR domain zinc finger protein 9, MEISETZ, and PR/SET domain 9, GenBank Accession No. NM_020227, a.a. 2-400, with an N-terminal GST-tag, expressed in an E. coli expression system. MW = 73 kDa.

●

Synonyms

PRDM9, PR domain zinc finger protein 9, MEISETZ, PR/SET domain 9

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PRDM9 inhibition curve using a chemiluminescent assay (#79625). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥46%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH8.0, 110 mM NaCl, 2.2 mM KCl, 20mM glutathione, 3mM DTT and 20% glycerol

73 kDa

Amino Acids

2-400

Specific Activity

4.86 pmol/min/µg

Genbank #

NM_020227

UniProt #

Q9NQV7

Tag(s)

N-terminal GST-Tag

References

1. Baudat, F., et al., Science. 2010 Feb; 327(5967): 836-40.
2. Parvanov, E., et al., Science. 2010 Feb; 317(5967): 835.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

For standard, H3, 1-21 K4 (2Me)-Biotin was coated onto a neutravadin plate at varying concentrations. For test lots, H3,1-21-Biotin was coated onto a neutravadin plate at 0.4 µg/ml. Reaction mixture contained 50 mM Tris buffer pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and various amounts of PRDM9—incubated for 1 hour at room temperature. Followed by 1 hour incubation with primary antibody against H3 K4 (2Me), and 30 minute incubation with secondary antibody. Luminescence was developed with HRP substrate A and B (Pierce).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Methyltransferase Enzyme

Regulatory Status

Research Use Only