METTL3/METTL14 Complex Chemiluminescent Assay Kit

Catalog #

79614

$1,865 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The METTL3/METTL14 Complex Chemiluminescent Assay Kit is designed to measure METTL3/METTL14 complex activity for screening and profiling applications. The key to the METTL3/METTL14 Complex Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6-methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against METTL3/METTL14 complex? Check out our Methyltransferase Screening Service.

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Synonyms

Methyltransferase-like 3, METTL3, RNA, METTL14

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The METTL3/METTL14 Complex Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated adenosine, a secondary HRP-labeled antibody, S-adenosylmethionine, methylt

Materials Required But Not Supplied

TBST buffer (1 x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Dithiothreitol (0.5 M DTT)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
100105	METTL3/METTL14 human enzyme	20 μg	-80°C	Avoid freeze/ thaw cycles!
52120	20 μM S-adenosylmethionine	250 μl	-80°C
52140Z4	Primary antibody 29	100 μl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 μl	-80°C
78368	4x METTL assay buffer*	3 ml	-20°C
	Blocking buffer 5	50 ml	+4°C
79670	ELISA ECL substrate A (translucent bottle)	6 ml	Room Temp.
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp.
	White microplate precoated with the RNA substrate**	1	+4°C

*Important: Use RNAse-free conditions!
**Add 30 μl of 0.5 M DTT.

UniProt #

"METTL3: Q86U44; METTL14: Q9HCE5"

References

Liu, J., et al. Nat Chem Biol. 2014; 10(2): 93–95.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only