FBXL11 (KDM2A) Homogeneous Assay Kit

Catalog #: 50611
Size: 384 reactions
$1,050
Background:
FBXL11, also known as KDM2A, JHDM1A, and Ndy2, is a histone lysine demethylase that exhibits demethylation activity toward histone H3 lysine 36.
Description:
The FBXL11 Homogeneous Assay Kit is designed to measure the activity of the FBXL11 for screening and profiling applications. The key to the FBXL11 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing FBXL11 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Synonym(s):
Lysine-specific demethylase 2A, KDM2A, JHDM1A, and Ndy2, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Supplied As:
The FBXL11 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified FBXL11 for 384 enzyme reactions.
Contraindications:
Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Format:

COMPONENTS:

Instructions for use:
See product datasheet for detailed instructions.
Storage / Stability:
Stable for at least one year from date of receipt when stored as directed. Be sure to store each component at the proper storage temperature.
Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):
Iwase, S., et al. Cell 2007; 128(6):1077-1088.
Notes:
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s):
Avoid freeze thaw cycles.
Scientific Category:
Demethylase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50611.pdf