JMJD1A (KDM3A) Homogeneous Assay Kit

Catalog #: 50412
Size: 384 reactions
$1,950
Description:
The JMJD1A Homogeneous Assay Kit is designed to measure activity of the JMJD1A for screening and profiling applications. JMJD1A, also known as KDM3A, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward monoand dimethyl-lysine 9 on histone H3. The key to the JMJD1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JMJD1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Synonym(s):
JMJD1A, KDM3A, JHDM2A
Supplied As:
The JMJD1A Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD1A for 384 enzyme reactions.
Contraindications:
Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Format:
Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):

Horton, J.R., et al. Nature Structural & Molecular Biology 2010; 17(1): 38-43.

Notes:

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Demethylases
Product Type:
Assay Kits
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50412_Lot_170712.pdf