VSV-G Pseudotyped VSV Delta G (Luciferase Reporter)
The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) was produced by re-expression of VSV-G as the envelope glycoprotein using the VSV Delta G system in which VSV-G is deleted. The pseudovirions contain the firefly luciferase gene; therefore, the VSV-G mediated cell entry can be measured via luciferase activity. The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) can be used as a positive control of transduction for other VSV pseudotypes containing the envelope glycoproteins of heterologous viruses in a Biosafety Level 2 facility.
| Name | Ordering Information |
| Thaw Medium 1 | BPS Bioscience #60187 |
| Spike (SARS-CoV-2) (BA.2, Omicron Variant) pseudotyped VSV Delta G (Luciferase Reporter) | BPS Bioscience #78635 |
| Vero-E6 | ATCC #CRL-1586 |
| ACE2-HEK293 Recombinant Cell Line | BPS Bioscience #79951 |
| Spike Neutralizing Antibody (Clone G10xA1) (SARS-CoV-2) | BPS Bioscience #101326 |
| Anti-VSV-G Antibody (Clone 8G5F11) | MilliporeSigma #MABF2337 |
| ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
| 96-well tissue culture treated, white clear-bottom assay plate | Corning #3610 |
The pseudoviruses were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Vesicular stomatitis virus (VSV) is an enveloped, negative-stranded RNA virus that infects a wide range of animals and less frequently humans, causing mild flu-like symptoms. Its simple structure and its ability to grow in most mammalian cell types has made VSV a valuable tool to study virus entry, replication, and assembly. The glycoprotein of VSV (VSV-G), which binds to the LDL-receptor (low-density lipoprotein receptor), is responsible for the attachment and entry of VSV into a susceptible host cell. Recombinant VSV in which the glycoprotein was deleted (VSV Delta G) can accept viral envelop proteins from a variety of other viruses, allowing to generate pseudotypes that represent robust models to screen for neutralizing antibodies and other inhibitors of virus entry. The pseudoviruses can be engineered to transduce a reporter gene such as Firefly Luciferase or a fluorescent protein, so that viral entry can be monitored using luminescence or fluorescence.
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