TrkA / SRE Reporter – HEK293 Recombinant Cell Line

Catalog #

79798

$9,400 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant HEK-293 cells expressing firefly luciferase gene under the control of Serum Response Elements (SRE) with constitutive expression of human TrkA (Tropomyosin receptor kinase A; NTRK1; ref. seq. NM_002529.3).

Interested in screening and profiling inhibitors, blocking antibodies, or activators of TrkA without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

TrkA, Tyrosine Kinase Receptor A, NTRK1, Tropomyosin receptor kinase A;

Nerve Growth Factor, NGFB, trk, trka, neurotrophic tyrosine kinase receptor, Trk-A

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Product Data Gallery

Dose response of TrkA/SRE-HEK293 cells to human NGF

Inhibition of TrkA by Larotrectinib (LOXO101) in TrkA/SRE-HEK293 cells

Inhibition of NGF/TrkA signaling by anti-NGF antibody in TrkA/SRE-HEK293 cells

FACS Analysis

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS

Materials Required But Not Supplied

Human NGF (R&D Systems, #256-GF-100)
Thaw Medium 1 (BPS Bioscience #60187), Growth Medium 1A (BPS Bioscience #79528), Assay Medium 1A (BPS Bioscience #79805)
96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
ONE-Step™ Luciferase Assay System (BPS Bioscience #60690)
Luminometer

Genbank #

NM_002529.3

UniProt #

P04629

Mycoplasma Testing

The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.

Background

A chronic pain mediator, NGF (Nerve Growth Factor) binds to TrkA resulting in activation of the downstream signaling pathway linked to pro-nociception; therefore, targeting the kinase activity of TrkA and the interaction between NGF/TrkA have been attractive tools in pain management research. In addition to control of chronic pain, a recent clinical success has proven that inhibition of TrkA kinase would be a promising anti-cancer strategy.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience, #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1A (BPS Bioscience, #79528): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 μg/ml of Geneticin and 100 μg/ml of Hygromycin B

Cells should be grown at 37°C with 5% CO2 using Growth Medium 1A.

Assay Medium 1A (BPS Bioscience #79805): Opti-MEM plus 1% Penicillin/Streptomycin

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway, Neuroscience

Regulatory Status

Research Use Only