TrkA Assay Kit

Catalog #

79548

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The tropomyosin receptor kinases (TRK) are a family of tyrosine receptor kinases, which include TrkA, TrkB and TrkC. TrkA is activated by binding to nerve growth factor (NGF), resulting in activation of cell proliferation through the RAS/MAPK/ERK and PLCγ/PI3K pathways. Constitutive activation of TrkA is associated with colorectal cancer, suggesting TrkA inhibitors may have potential therapeutic value. The TrkA Assay Kit is designed to measure TrkA activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against TrkA? Check out our Kinase Screening Services.

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Synonyms

trk, MTC, Trk1, Trk-A, NTRK1, Trk-A

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The TrkA Assay Kit comes in a convenient 96-well format, with enough purified recombinant TrkA enzyme, TrkA substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Reagent	Amount	Storage
40280	TrkA	10 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79334	5x Kinase assay buffer 1	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	PTK substrate Poly (Glu: Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp

UniProt #

P04629

Storage/Stability

Up to 6 months when stored as recommended

Instructions for Use

See data sheet for detailed assay instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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TrkA Assay Kit

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