TRKA, GST-tag Recombinant

Catalog #

40280

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human TRKA, also known as NTKR1, and Neurotrophic Tyrosine Kinase Receptor, type 1, GenBank Accession No. NM_002529, a.a. 442-796(end), with Nterminal GST-tag, expressed in Sf9 cells via a baculovirus expression system. MW=65 kDa.

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Synonyms

TRKA, NTKR1, Neurotrophic Tyrosine Kinase Receptor, type 1, Trk-A

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl,0.04% Tween20, 3mM DTT, and 20% glycerol

65 kDa

Amino Acids

442–796(end)

Specific Activity

100 pmole/min/μg

Genbank #

NM_002529

UniProt #

P04629

Tag(s)

N-terminal GST-tag

References

1. Koch, A., et al. (2000). FEBS Lett. 469 (1): 72–62.
2. Wiesmann, C., et al. (1999). Nature 401 (6749): 184–83.
3. Yamashita, H. et al. (1999). J. Biol. Chem. 274 (21): 15059–65

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml Poly(Glu:Tyr) substrate and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40280 Lot 151113-G.pdf

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TRKA, GST-tag Recombinant

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