TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line
TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line is a Jurkat cell line where TCR (T Cell Receptor) was knockout. The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells stably expressing the firefly luciferase reporter under the control of NFAT response elements.
This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to the parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621).
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Media Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 2 | BPS Bioscience #60184 |
| Growth Medium 2B | BPS Bioscience #79530 |
Materials Used in the Cellular Assay
| Name | Ordering Information |
| Thaw Medium 2 | BPS Bioscience #60184 |
| NFAT-Luciferase Reporter Jurkat cells | BPS Bioscience #60621 |
| Anti-CD3 Agonist Antibody | BPS Bioscience #71274 |
| ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
| Luminometer | |
| 96-well tissue culture plate, white, clear bottom |
The cell line has been screened to confirm the absence of Mycoplasma species.
The TCR (T Cell Receptor) is found on the surface of T cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR results in activation of downstream NFAT (Nuclear factor of activated T-cells) signaling. NFAT is a family of transcription factors that has an important function in immune responses, for example by inducing the expression of various cytokines (such as interleukin-2 to 4, and TNF-alpha) in T cells. NFAT is regulated by Ca2+ and the Ca2+/calmodulin-dependent serine phosphatase, calcineurin.
The TCR consists of a heterodimer of two different protein chains, of which the alpha (α) and beta (β) chains are the predominant chains. CRISPR/Cas9 genome editing was used to remove the TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) regions of the α and β chains, resulting in loss of TCR expression.
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