TCR CRISPR/Cas9 Lentivirus (Integrating)

Catalog #

78055

$795 *

Size: 500 µl x 2

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The T Cell Receptor (TCR) is found on the surface of T-cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Activation of the TCR results in activation of downstream NFAT signaling. The TCR consists of a heterodimer of two different protein chains, of which the alpha (α) and beta (β) chains are the predominant chains.

The TCR CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1A promoter, along with 4 sgRNA (single guide RNA) targeting human TRAC (T-Cell Receptor Alpha Constant) and human TRBC1 (T-Cell Receptor Beta Constant 1) regions of the α and β chains.

The DNA transduced by the integrating lentivirus integrates randomly into the cellular genome to express both Cas9 and sgRNA. Puromycin selection increases the knockout efficiency by forcing high expression levels of both Cas9 and the sgRNA, and can be used with the integrating lentivirus to quickly and easily achieve high knockdown efficiencies in a cell pool. Efficiencies also depend on the cell type and the gene of interest.

Figure 1: Schematic of the Lenti-vector used to generate the TCR CRISPR/Cas9 Lentivirus.

Gene Target	Primer ID:	sgRNA Sequence
TRAC	TCR-1	AGAGTCTCTCAGCTGGTACA
TRAC	TCR-2	TGTGCTAGACATGAGGTCTA
TRBC1	TCR-3	GGAGAATGACGAGTGGACCC
TRBC1	TCR-4	GCAGTATCTGGAGTCATTGA

Figure 2: List of sgRNA Sequences in the TCR CRISPR/Cas9 Lentivirus.

●

Synonyms

SpCas9 lentivirus, CRISPR lentivirus, Cas9 lentivirus, T-Cell Receptor CRISPR

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Replication incompetent HIV

Supplied As

Two vials (500 μl x 2) of lentivirus at a titer ≥1 x 10⁶ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Transient knock-down of TCR in target cells.
Generation of a stable TCR knock-out cell line following puromycin selection.

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

Biosafety
The lentiviruses are produced with the SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Warnings

Avoid repeated freeze-thaw cycles.

Scientific Category

CRISPR

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

TCR CRISPR/Cas9 Lentivirus (Integrating)

PRODUCTS

SERVICES

RESOURCES

COMPANY