ROS1, GST-His-tag Recombinant

Catalog #

40268

$365 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human ROS1 (ROS proto-oncogene 1), encompassing amino acids 1883-2347 (end) that contain the tyrosine kinase domain. The N-terminal end of this construct contains a GST-tag followed by a His-tag (6xHis), linked to the protein via a linker that includes a thrombin cleavage site, allowing removal of the tags from the protein if desired. The protein was affinity purified.

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Synonyms

ROS, c-Ros Oncogene 1, MCF3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, 2 mM glutathione

82 kDa

Amino Acids

1883–2347(end)

Specific Activity

60 pmol/min/μg

Genbank #

NM_002944

UniProt #

P08922

Tag(s)

N-terminal GST-His-tag

References

1. Charest, A., et al. (2003). Proc. Natl. Acad. Sci. USA 100 (3): 916–21.
2. Sharma, S., et al. (1990). Oncogene Res. 5 (2): 91–100.
3. Keilhack, H., et al. (2001). J. Cell Biol. 152 (2): 325–34.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done according to the HPK1 Assay Kit (BPS Bioscience # 79775) with ROS-1 titrated from 800 ng/rxn – 12.5 ng/rxn, 20 µM ATP was used instead of 10 µM, and IGFR-1tide at 0.1 mg/ml used instead of MBP.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40268 Lot 200911-1

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