HPK1 Assay Kit

Catalog #

79775

$560 *

Size: 96 reactions

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The HPK1 Assay Kit is designed to measure HPK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.

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Synonyms

MAP4K1, Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1, Hematopoietic Progenitor Kinase 1, MAPK/ERK Kinase Kinase Kinase 1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Species

Human

Supplied As

The HPK1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant HPK1 enzyme, HPK1 substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Components	Amount	Storage
40398	HPK1	3 µg	-80°C	*Avoid freeze/ thaw cycles!*
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
	MBP (5 mg/ml)	200 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

Q92918

Background

HPK1 (MAP4K1) or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. It is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. HPK1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76, suggesting HPK1 could be a novel target for anti-tumor immunotherapy.

References

1. Wu P, et al., Structure, 2019, 27: 125-133.e4
2. Alzabin S, et al., J. Immunol. 2009, 182: 6187-6194
3. Jakob SM, et al., Blood, 2013, 121(20):4184-4194

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HPK1 Assay Kit

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