HPK1, GST-Tag, His-Avi-Tag (Human) Recombinant

Catalog #

40398

$390 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human HPK1 (also known as MAP4K1, Mitogen-activated protein kinase kinase kinase kinase 1), encompassing amino acids 1-346 containing the kinase domain of the protein. In this construct the N-terminal end is fused with GST, linked to the protein via a short peptide linker corresponding to a thrombin cleavage site. The construct also contains a C-terminal His tag (6xHis) followed by an Avi-Tag™. The protein was affinity purified.

●

Synonyms

HPK1 protein, hematopoietic progenitor kinase 1, mitogen activated protein kinase 1 MAP4K1, HPK1 protein, HPK, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

HPK1 (GST-Th-1-346-His-Avi)

Host Species/Expression System

Sf9 Insect Cell

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol

67 kDa

Amino Acids

1-346

Specific Activity

≥100 pmol/min/µg

Genbank #

NM_007181

UniProt #

Q92918

Tag(s)

N-terminal GST tag, C-terminal His-Avi-Tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to the HPK1 Assay Kit (BPS Bioscience #79775) with HPK1 titrated from 120 ng/rxn – 1.875 ng/rxn. 20 µM ATP was used instead of 10 µM.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40398 Lot 210709

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

HPK1, GST-Tag, His-Avi-Tag (Human) Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY