PIM3 Assay Kit
The PIM3 Assay Kit is designed to measure PIM3 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The PIM3 Assay Kit comes in a convenient 96-well format, with enough purified recombinant PIM3 enzyme, PIM substrate (S6Ktide), ATP, and kinase assay buffer for 100 enzyme reactions.
Need us to run inhibitor screens or profile your compounds against PIM3? Check out our Kinase Screening Services.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Catalog Number |
Reagent |
Amount |
Storage |
|
41108 | PIM3* | 2.5 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
79686 | ATP (500 μM) | 100 µl | -20°C | |
79884 | PIM substrate (S6Ktide, 10 mg/ml) |
100 µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp. |
*The concentration of PIM3 is lot-specific and will be indicated on the tube containing the enzyme.
PIM kinases (PIM1, PIM2 and PIM3) are a family of serine/threonine protein kinases that play crucial roles in cell survival, proliferation, and drug resistance. PIM kinases are overexpressed in several tumors and promote growth and survival of malignant cells through cell cycle regulation and/or inhibition of apoptosis. Recently, PIM kinases were identified as a potential therapeutic target for precision medicine of advanced cancer.
1. Jeyapal G. P., et al., Anticancer Agents Med Chem, 2018, 18(8): 1100-1114
2. Asati V., et al., Eur J Med Chem. 2019, 172: 95-108