PIM1, GST-tag Recombinant

Catalog #

41107

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Synonyms

serine/threonine-protein kinase PIM1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 Insect Cells

Purity

>90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, 2 mM glutathione

62 kDa

Amino Acids

full-length

Specific Activity

≥150 pmol/min/µg

Genbank #

NM_002648

UniProt #

P11309

Tag(s)

N-terminal GST-Tag

Background

PIM1 is a proto-oncogene that belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, PIM1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others (1). Expression of PIM1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational (2). Expression of PIM1 is mediated through activation of the JAK/STAT pathway.

References

1. Meeker, T.C., et al., J. Cell. Biochem. 1987; 35(2): 105-112.
2. Friedmann, M. et al., Arch. Biochem Biophys. 1992; 298(2): 594-601.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml S6Ktide substrate and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

Dry ice

Scientific Category

Kinase - Serine/Threonine

Regulatory Status

Research Use Only