PIM1 Assay Kit

Catalog #

79885

$560 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PIM1 Assay Kit is designed to measure PIM1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against PIM1? Check out our Kinase Screening Services.

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Synonyms

Pim-1 proto-oncogene assay kit, serine/threonine kinase assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The PIM1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant PIM1 enzyme, PIM substrate (S6Ktide), ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
41107	PIM1	2.5 µg	-80°C	*Avoid* *multiple* *freeze/* *thaw* *cycles!*
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
79884	PIM substrate (S6Ktide, 10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P11309

Background

PIM kinases (PIM1, PIM2 and PIM3) are a family of serine/threonine protein kinases that play crucial roles in cell survival, proliferation, and drug resistance. PIM kinases are overexpressed in several tumors and promote growth and survival of malignant cells through cell cycle regulation and/or inhibition of apoptosis. Recently, PIM kinases were identified as a potential therapeutic target for precision medicine of advanced cancer.

References

1. Jeyapal G. P., et al., Anticancer Agents Med Chem, 2018, 18(8): 1100-1114
2. Asati V., et al., Eur J Med Chem. 2019, 172: 95-108

Application Reference(s):

1. Data Sheet PIM1 Assay Kit Catalog #79885 96 Reactions (2020)

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only