PIM2 Assay Kit
The PIM2 Assay Kit is designed to measure PIM2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Need us to run inhibitor screens or profile your compounds against PIM2? Check out our Kinase Screening Services.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Catalog Number |
Reagent |
Amount |
Storage |
|
40153 | PIM2 | 2.5 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
79686 | ATP (500 μM) | 100 µl | -20°C | |
79884 | PIM substrate (S6Ktide, 10 mg/ml) |
100 µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp. |
PIM kinases (PIM1, PIM2 and PIM3) are a family of serine/threonine protein kinases that play crucial roles in cell survival, proliferation, and drug resistance. PIM kinases are overexpressed in several tumors and promote growth and survival of malignant cells through cell cycle regulation and/or inhibition of apoptosis. Recently, PIM kinases were identified as a potential therapeutic target for precision medicine of advanced cancer.
1. Jeyapal G. P., et al., Anticancer Agents Med Chem, 2018, 18(8): 1100-1114
2. Asati V., et al., Eur J Med Chem. 2019, 172: 95-108