NEK6 Kinase Assay Kit
The NEK6 Kinase Assay Kit is designed to measure NEK6 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.
ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog Number |
Reagent |
Amount |
Storage |
|
| 40014 | NEK6, His-tag | 10 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
| 79686 | ATP (500 μM) | 100 µl | -20°C | |
| MBP (5 mg/ml) | 50 µl | -20°C | ||
| 79696 | 96-well plate, white | 1 | Room Temp |
|
NEK6 is a protein kinase in the NIMA-related serine/threonine kinase family that plays a role in mitotic cell cycle progression. Overexpression of NEK6 has been linked to many human diseases including liver, breast, lung, stomach, colon, larynx, ovary and prostate cancer, making NEK6 an attractive target for anti-cancer therapeutics. Inhibition of NEK6 has been shown to induce cell death and premature senescence.
Yin, M.-J., et al. 2003. "The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis." J. Biol. Chem. 278(52): 52454-52460.
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