Myc Luciferase Reporter Lentivirus

Catalog #

78628

$835 *

Size: 500 µl x 2

Qty

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Description

The Myc Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Myc response element located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the Myc signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Figure 1. Schematic of the lenti-vector used to generate the Myc luciferase reporter lentivirus.

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Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer >10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
HCT116 cells	ATCC #CCL-247
ICG-001	Selleckchem #S2662
Thaw Medium 7	BPS Bioscience #60185
Assay Medium 7B	BPS Bioscience #79718
Polybrene	Millipore, #TR-1003-G
96-well tissue culture, clear-bottom, white plate	Corning, #3610
One-Step luciferase assay system	BPS Bioscience #60690
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Formulation

The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Background

The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. c-Myc is a transcription factor that heterodimerizes with Max to regulate Myc signaling pathway-responsive genes. Genetic alterations in MYC have been linked to a number of human cancers, including Burkitt’s lymphoma, cervical, ovarian, breast, lung and pancreatic carcinomas. Thus, Myc is a promising therapeutic target for cancer treatment. Myc can be activated by the Wnt/β-catenin pathway.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 7 (BPS Bioscience, #60185): McCoy’s 5A medium with 10% FBS, 1% Penicillin/Streptomycin.

Assay Medium 7B (BPS Bioscience, #79718): Opti-MEM I + 0.5% FBS + 1% non-essential amino acids + 1 mM sodium pyruvate + 1% penicillin/ streptomycin.

Applications

Screen for activators or inhibitors of Myc signaling pathway in transduced target cells
Generate Myc Luciferase Reporter stable cell line (puromycin resistant)

Shipping Temperature

-80°C

Notes

Biosafety
The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which
ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Myc Luciferase Reporter Lentivirus

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