Lumi-Verse™ VEGFR2 (KDR) Kinase Assay Kit
The Lumi-Verse™ VEGFR2 (KDR) Kinase Assay Kit is designed to measure Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) tyrosine kinase activity for screening and profiling applications, using the highly sensitive ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant VEGFR2 (KDR) kinase domain (amino acids 805-1356), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
- ADP-Glo™ Kinase Assay (Promega #V6930)
- DTT (Dithiothreitol), 1 M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40301 | VEGFR2 (KDR), aa 805-1356, GST-Tag* | 2 µg | -80°C |
| 79334 | 5x Kinase Buffer 1 | 1.5 ml | -20°C |
| 79686 | 500 µM ATP | 50 µl | -20°C |
| 40217 | PTK Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) | 50 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
Vascular endothelial growth factor receptor 2 (VEGFR2), also known as Kinase insert Domain receptor (KDR), is a tyrosine kinase receptor for the growth factor VEGF. EGFR (epidermal growth factor receptor) overexpression is a common feature in many cancer types, such as breast and ovarian cancer. EGFR activation results in VEGF expression and signaling via VEGFR2, promoting angiogenesis and tumor progression. The inhibition of VEGFR2 via small molecules or antibodies, alone or in combination with inhibitors of EGFR, are promising therapeutic strategies for cancer therapy.
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