LTK, GST-Tag Recombinant

Catalog #

101357

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human LTK (leukocyte receptor tyrosine kinase, also known as TYK1), encompassing amino acids 498-796 containing the protein kinase domain. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is kinase-active.

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Synonyms

Leukocyte receptor tyrosine kinase, TYK1

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

LTK (GST-498-796)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 PMSF, 25% glycerol

70 kDa

Amino Acids

498-796

Specific Activity

15 pmol/min/µg

Genbank #

NM_002344

UniProt #

P29376

Tag(s)

N-terminal GST-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The activity of the complex was measured using peptide sequence (EAIYAAPFAKKK), based on the C-terminus of Abl, as a substrate (stock solution 1 mg/ml dissolved in distilled water).
Assay: Increasing amounts of kinase were mixed with a final concentration of 200 µg/ml peptide substrate in a buffer consisting of 15 mM MOPS pH 7.2, 15 mM MgCl2, 7.5 mM β-glycerol-phosphate, 3 mM EGTA, 1.2 mM EDTA, 20 µg/ml BSA (bovine serum albumin) and 150 µM fresh DTT (final concentrations). The reaction was initiated by adding a [33P]-ATP (1 µCi/sample) mixed with non-radioactive ATP to reach a final concentration of 50 µM. The blank was determined from a “no substrate” sample.
The reaction was incubated for 15min at 30°C and terminated by spotting 20 μl of the mixture onto phosphocellulose paper strips that were fixed in 1% phosphoric acid and washed three times. Radioactivity was determined using a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

101357 Lot 220127

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