JAK3, His-tag Recombinant

Catalog #

40452

$375 *

Size: 20 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human JAK3 (Janus kinase 3), also known as LJAK, GenBank Accession No. NM_000215, a.a. 811-1124(end) with N-terminal His-tag, MW= 37 kDa, expressed in a baculovirus expression system.

●

Synonyms

JAK3, Janus kinase-3, Just Another Kinase 3, LJAK, jak

●

Product Data Gallery

Specific Activity

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

37 kDa

Amino Acids

811–1124(end)

Specific Activity

500 pmol/min/μg.

Genbank #

NM_000215

UniProt #

P52333

Tag(s)

N-terminal His-tag

References

1. Kim S. et al., Cell. Signal. 21 (6), 892-898 (2009).
2. Mullighan C.G. et al., Proc. Natl. Acad. Sci. U.S.A. 106 (23), 9414-9418 (2009).

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr) substrate and 20 mM ATP. Reaction was done at 30°C for 45 min.

Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

JAK3, His-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY