Histone H3, Full Length, Biotin-labeled, His-tag
Catalog #
52047
$465
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Description
Recombinant human Histone H3 encompassing amino acids 2-136 (end). This construct contains an additional cysteine in N-terminal, followed by a His-tag (6xHis), and also contains mutations C97A and C111A. The protein was biotinylated and affinity purified.
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Synonyms
Histone 3.1, HIST1H3E, Histone 3.1, HIST1H3E, H3 clustered histone 6 (H3C6)
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Product Info
Storage and Usage
Citations
Species
Human
Construct
Histone H3 (C97A, C111A) (MAC-His-2-136(end))-(Biotin)
Mutation
C97A, C111A
Host Species/Expression System
E. coli
Purity
≥90%
Format
Aqueous buffer solution
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 3 mM DTT.
MW
16 kDa
Amino Acids
2–136(end)
Genbank #
NM_003532
UniProt #
P68431
Label
Histone H3 was modified to contain an N-terminal Met-Ala-Cys, and two additional mutations (C97A, C111A) removed the protein cysteine residues. This ensures site-specific biotinylation at the N-terminal cysteine. Biotin was added chemically to this free cysteine.