Anti–H3K18ac polyclonal antibody

Catalog #

25236

$415 *

Size: 100 µl

Qty

Bulk/Custom

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Description

Polyclonal antibody raised in rabbit against histone H3 containing the acetylated lysine 18 (H3K18ac), using a KLH–conjugated synthetic peptide

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

Acetylation of histone H3 is associated with active gene transcription. Acetylation at H3K18 and H3K24 favors methylation at H3R17me by CARM1/PRMT4. SIRT7 is a chromatin–associated H3K18Ac–specific deacetylase. Studies show a significant correlation between low expression of H3K18ac and better prognosis of patients with oesophageal squamous cell carcinoma.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K18ac (Cat. No. 25236). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,800.

Cross reactivity test using the antibody directed against H3K18ac
A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K18ac (Cat. No. 25236) with with peptides containing other modifications and unmodified sequences of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

Western blot analysis using the antibody directed against H3K18ac
HeLa cells were treated with butyrate and histone extracts (15 μg) were analysed by Western blot using the antibody against H3K18ac (Cat. No. 25236) diluted 1:250 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows a coomassie blue staining of the gel. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

Applications

ELISA (1:100)
DB (1:1000)
WB (1:250)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–H3K18ac polyclonal antibody

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