Anti–H3K9ac monoclonal antibody

Catalog #

25265

$415 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Monoclonal antibody raised in mouse against histone H3 acetylated at lysine 9 (H3K9ac), using a KLH–conjugated synthetic peptide

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Product Info

Storage and Usage

Citations

Isotype

IgG1

Clonality

monoclonal

Host Species/Expression System

Mouse

Purification

Protein A/G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300

Concentration

50 µg/50 µl

Immunogen

synthetic peptide

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Cross reactivity of the monoclonal antibody directed against H3K9ac
To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K9ac (Cat. No. 25265). The wells were coated with peptides containing the unmodified H3K9 region as well as the acetylated H3K9 and the acetylated H3K27. Figure 1 shows a high specificity of the antibody for the peptide containing the modification of interest.

Immunofluorescence using the monoclonal antibody directed against H3K9ac
HeLa cells were stained with the antibody against H3K9ac (Cat. No. 25265) and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9ac antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Applications

ChIP/Chip – seq (1 – 5 µg/ChIP)
ELISA (1:3000)
WB (1:1000 – 1:2000)
IF (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Datasheets/Protocols

25265_Lot150403.pdf

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Anti–H3K9ac monoclonal antibody

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