MMP11 Fluorogenic Assay Kit

Catalog #

78020

$975 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Fluorogenic MMP11 Assay Kit is designed to measure MMP11 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Need us to run inhibitor screens or profile your compounds against MMP11? Check out our Protease Screening Services.

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Synonyms

Stromelysin-3 kit, SL-3 kit, SL3 kit, matrix metalloproteinase 11 kit, MMP-11

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Supplied As

The kit comes in a convenient 96-well format, with purified MMP11 enzyme, fluorogenic substrate, and MMP assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading λexc/λem=328 nm/393 nm

Format

Catalog Number	Component	Amount	Storage
	MMP11	20 µg	-80°C	Avoid freeze/ thaw cycles!
79919	MMP Substrate (1 mM)	10 µl	-80°C
79917	1X MMP Assay Buffer 1	25 ml	-20°C
79685	Black, low binding black microtiter plate	1	Room Temp

UniProt #

P24347

Background

MMP11 (matrix metalloproteinase 11), also known as Stromelysin-3 (SL-3), is a member of the matrix metalloproteinase (MMP) family involved in the degradation of the extracellular matrix. MMP11 was initially discovered in breast carcinoma and has since been identified in diverse carcinomas and developmental processes. MMP-11 promotes cancer development by inhibiting apoptosis as well as enhancing migration and invasion of cancer cells. MMP11 is activated by furin, an enzyme linked to the entry of the SARS-CoV-2 virus into cells.

References

1. Peruzzi, Daniela, et al. 2009. "MMP11: a novel target antigen for cancer immunotherapy." Clinical Cancer Research 15(12): 4104-4113.
2. Cheng, Chun-Wen, et al. 2010. "The clinical implications of MMP-11 and CK-20 expression in human breast cancer." Clinica Chimica Acta 411(3-4): 234-241.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only