MMP13 Fluorogenic Assay Kit

Catalog #

79991

$465 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Fluorogenic MMP13 Assay Kit is designed to measure MMP13 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Need us to run inhibitor screens or profile your compounds against MMP13? Check out our Protease Screening Services.

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Synonyms

collagenase 3 kit, matrix metalloproteinase 13 kit, MMP-13

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Supplied As

The kit comes in a convenient 96-well format, with purified MMP13 enzyme, fluorogenic substrate, and MMP assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading λexc/λem=328 nm/393 nm

Format

Catalog Number	Component	Amount	Storage
11345	MMP13, His-tag	4 µg	-80°C	Avoid freeze/ thaw cycles!
79919	Fluorogenic MMP Substrate (1 mM)	10 µl	-80°C
79917	1X MMP Assay Buffer 1	25 ml	-20°C
79685	Black, low binding black microtiter plate	1	Room Temp

UniProt #

P45452

Background

MMP13 (matrix metalloproteinase 13), also known as collagenase 3, is a member of the matrix metalloproteinase (MMP) family involved in the degradation of the extracellular matrix. MMP13 primarily cleaves type II collagen, and it is expressed in the skeleton where it plays a role in restructuring the collagen matrix for bone mineralization and cartilage turnover. MMP13 is also overexpressed in rheumatoid arthritis, osteoarthritis, and many human carcinomas, suggesting its potential as a therapeutic target.

References

1. Wang, M., et al. MMP13 is a critical target gene during the progression of osteoarthritis. Arthritis Res Ther 15: R5 (2013).
2. Li, H., et al. New insights on the MMP-13 regulatory network in the pathogenesis of early osteoarthritis. Arthritis Res Ther 19: 248 (2017).

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only