DPP4 Assay Kit

Catalog #

80204

$465 *

Size: 96 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The Fluorogenic DPP4 Assay Kit is designed to measure DPP4 activity using purified DPP4 for screening and profiling applications. The key to the Fluorogenic DPP4 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP4 reactions; no time-consuming washing steps are required. The DPP fluorometric substrate is incubated with a sample containing DPP4 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against DPP4? Check out our Protease Screening Services.

This product has been cited 3 times.

●

Synonyms

DPPIV, CD26, Dipeptidylpeptidase 4, ADCP2, DPP

●

Product Data Gallery

Product Info

Storage and Usage

Citations3

Assay Kit Format

Fluorogenic

Supplied As

Supplied in a convenient 96-well format, with purified DPP4 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.

Format

Catalog Number	Component	Amount	Storage
80040	DPP4 human recombinant enzyme	1 µg	-80°C	Avoid freeze/ thaw cycles!
80300	DPP assay buffer	10 ml	-20°C
80305	Fluorogenic DPP substrate 1 in DMSO (0.5 mM)	100 µl	-80°C
	AMC Fluorescent standard (50 µM)	500 µl	-20°C
79685	black, low binding NUNC black microtiter plate	1 plate	Room temp.

UniProt #

P27487

Background

Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP4 plays a key role in glucose metabolism, immune regulation, signal transduction and apoptosis.

References

1. Deacon CF, Carr RD, and Holst JJ Front Biosci. 2008 Jan 1;13:1780-94.
2. Langley AK, Suffoletta TJ, and Jennings HRPharmacotherapy. 2007 Aug;27(8):1163-80.

Storage/Stability

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only