MMP2 Fluorogenic Assay Kit

Catalog #

79918

$465 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Fluorogenic MMP2 Assay Kit is designed to measure MMP2 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Need us to run inhibitor screens or profile your compounds against MMP2? Check out our Protease Screening Services.

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Synonyms

MMP-2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Supplied As

The kit comes in a convenient 96-well format, with purified MMP2 enzyme, fluorogenic substrate, and MMP2 assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading exc/em=328 nm/393 nm

Format

Catalog Number	Component	Amount	Storage
80213	Recombinant Human MMP2	1 µg	-80°C	Avoid freeze/ thaw cycles!
79919	1 mM MMP Substrate	10 µl	-80°C
79920	1X MMP2 Assay Buffer	25 ml	-20°C
79685	Black, low binding black microtiter plate	1	Room Temp

UniProt #

P08253

Background

MMP2 (matrix metalloproteinase 2), also known as Gelatinase A, is a member of the matrix metalloproteinase (MMP) family involved in the breakdown of type IV collagen, a major structural component of basement membranes. The activity of MMP2 is associated with cancer cell metastasis and other diseases, including Multicentric Osteolysis, Nodulosis, and Arthropathy.

References

1. Björklund, M. and Koivunen, E. 2005 May 25; Biochimica et Biophysica Acta. 1755 (1): 37–69.
2. Olaf, R.F., et al. 2004 Dec 17; Biochimica et Biophysica Acta. 1705 (2): 69–89.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See data sheet for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only