FLT1 Kinase Assay Kit
The FLT1 Kinase Assay Kit is designed to measure FLT1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog Number |
Reagent |
Amount |
Storage |
|
| 40223 | FLT1 | 10 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
| 79686 | ATP (500 μM) | 100 µl | -20°C | |
| 40217 | Poly-(Glu,Tyr 4:1) (10 mg/ml) | 50 µl | -20°C | |
| 79698 | 96-well plate, white | 1 | Room Temp |
|
FLT1 is a protein kinase in the src gene family that plays a role in mitotic cell cycle progression. Alteration of FLT1 has been shown in various cancers such as breast carcinoma and melanoma.
1. Autiero, M., et al. 2003. "Role of PlGF in the intra-and intermolecular cross talk between the VEGF receptors Flt1 and Flk1." Nature Medicine 9(7): 936-943.
2. Kaipainen, A., et al. 1993. "The related FLT4, FLT1, and KDR receptor tyrosine kinases show distinct expression patterns in human fetal endothelial cells." The Journal of Experimental Medicine 178(6): 2077-2088.
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