FLT1 Kinase Assay Kit

Catalog #
78019
$535 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The FLT1 Kinase Assay Kit is designed to measure FLT1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent. 

Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Species
Human
Supplied As
The FLT1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant FLT1 enzyme, Poly-(Glu,Tyr 4:1), ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format
Catalog
Number

Reagent

Amount

Storage
40223 FLT1 10 µg -80°C

Avoid
multiple
freeze/
thaw
cycles!
79334 5x Kinase assay buffer 1.5 ml -20°C
79686 ATP (500 μM) 100 µl -20°C
40217 Poly-(Glu,Tyr 4:1) (10 mg/ml) 50 µl -20°C
79698 96-well plate, white 1 Room
Temp
 
UniProt #
P17948
Background

FLT1 is a protein kinase in the src gene family that plays a role in mitotic cell cycle progression. Alteration of FLT1 has been shown in various cancers such as breast carcinoma and melanoma.

References

1. Autiero, M., et al. 2003. "Role of PlGF in the intra-and intermolecular cross talk between the VEGF receptors Flt1 and Flk1." Nature Medicine 9(7): 936-943.
2. Kaipainen, A., et al. 1993. "The related FLT4, FLT1, and KDR receptor tyrosine kinases show distinct expression patterns in human fetal endothelial cells." The Journal of Experimental Medicine 178(6): 2077-2088.