eGFP/Firefly Luciferase JeKo-1 Cell Line

Catalog #

82147

$3,250 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The eGFP/Firefly Luciferase JeKo-1 Cell Line is a JeKo-1 cell line expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter, generated by transduction with Firefly Luciferase-eGFP lentivirus (BPS Bioscience #78741), which is a SIN0 self-inactivating lentivirus).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity and eGFP expression in GFP/Firefly Luciferase JeKo-1 Cell Line

Expression of eGFP in the GFP/Firefly Luciferase JeKo-1 Cell Line by flow cytometry

Product Info

Storage and Usage

Citations

Host Cell Line

Jeko-1 cells, Peripheral blood, Lymphoblast, Suspension

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2M	BPS Bioscience #78181

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The JeKo-1 mantle cell lymphoma (MCL) cell line was isolated from the peripheral blood mononuclear cells of a 78-year-old female with a large cell variant of MCL showing leukemic conversion. The JeKo-1 cells are highly tumorigenic in SCID mice and have applications in immunology research. These cells overexpress cyclin D1, Bcl-2 (B-cell lymphoma 2), c-Myc and Rb proteins. A Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The presence of eGFP and luciferase allow for easy assay readouts, making this cell line a convenient choice.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2M (BPS Bioscience #78181): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 µg/ml of Puromycin

Applications

Use as target cells in CAR-T or NK co-culture killing assays.
In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only