DR3:TL1A Inhibitor Screening Assay Kit
The DR3:TL1A Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the interaction between DR3 (Death Receptor 3) and TL1A (TNF-like ligand 1A). This kit comes in a convenient 96-well or 384-well format, with biotin-labeled TL1A, purified DR3, streptavidin-labeled HRP, and assay buffer for 100 or 400 binding reactions.
Figure 1: DR3:TL1A Inhibitor Screening Assay Kit workflow diagram.
First, DR3 is coated on a 96-well or 384-well plate. Next, biotinylated TL1A is incubated with DR3. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence. The chemiluminescence signal is proportional to the binding of DR3:TL1A
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- 1x PBS (Phosphate Buffer Saline)
- PBST Buffer (1x PBS containing 0.05% Tween-20)
- Plate reader capable of reading chemiluminescence
- Adjustable micropipettor and sterile tips
- Orbital shaker
96 Reactions
| Catalog # | Name | Amount | Storage |
| 101882 | TL1A, His-Tag, Avi-Tag, Biotin-Labeled* | 5 µg | -80 °C |
| DR3* | 25 µg | -80 °C | |
| 5x PP-02 Buffer | 4 ml | -20 °C | |
| Blocking Buffer 7 | 40 ml | +4 °C | |
| 79742 | Streptavidin-HRP | 10 µl | +4 °C |
| 79670 | ELISA ECL Substrate A (translucent bottle) | 6 ml | Room Temp |
| ELISA ECL Substrate B (brown bottle) | 6 ml | Room Temp | |
| 79699 | 96-well white microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
384 Reactions
| Catalog # | Name | Amount | Storage |
| 101882 | TL1A, His-Tag, Avi-Tag, Biotin-Labeled* | 2 x 5 µg | -80 °C |
| DR3* | 2 x 25 µg | -80 °C | |
| 5x PP-02 Buffer | 4 ml | -20 °C | |
| Blocking Buffer 7 | 2 x 40 ml | +4 °C | |
| 79742 | Streptavidin-HRP | 2 x 10 µl | +4 °C |
| 79670 | ELISA ECL Substrate A (translucent bottle) | 2 x 6 ml | Room Temp |
| ELISA ECL Substrate B (brown bottle) | 2 x 6 ml | Room Temp | |
| 78188 | 384-well white microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
DR3 (death receptor 3), also known as tumor necrosis factor receptor superfamily member 25 or TNFRSF25, is a membrane receptor of the tumor necrosis factor receptor superfamily of proteins (TNFRSF), which associates with TL1A (TNF-like protein 1A) in T and NK cells. DR3 has been recognized as a significant anti-apoptotic and differentiation factor and it is a co-stimulatory receptor. TL1A, also called TNFSF15, is a member of the tumor necrosis factor family. It is expressed in different immune cells, such as monocyte, macrophage, dendritic cell, T cell and non-immune cells. TL1A competitively binds to DR3, having a higher affinity for DcR3 (decoy receptor 3), providing stimulatory signal for downstream signaling pathways. It then regulates proliferation, activation, apoptosis, and chemokine production in effector cells. The role of DR3 in T cell activation, and consequently in cytokine secretion and cell proliferation, makes it an attractive target in cancer therapy. Inhibition of DR3-TL1A interaction has substantial therapeutic potential in the treatment of solid tumors.
Xu, W. D., et al., 2022 Front. Immunol. 13: 1-10.
Zwolak, A., et al., 2022 Sci. Rep. 12(1): 20538.
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