This anti-TL1A (TNF-like ligand 1A) antibody is a purified, recombinant human monoclonal antibody which recognizes the human TL1A protein. TL1A is also known as vascular endothelial growth inhibitor, VEGI and TNFSF15 (Tumor Necrosis Factor Super Family 15).
Protein A affinity chromatography from HEK293 supernatants.
Format
Aqueous buffer solution.
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
MW
Heavy Chain: 49 kDa + glycans; Light Chain: 23 kDa
Glycosylation
This antibody runs at a higher MW by SDS-PAGE due to glycosylation.
UniProt #
O95150
Background
TL1A is involved in immune regulation by binding to two receptors of the TNF (Tumor Necrosis Factor) family present in T and NK cells: DR3 and DcR3. Soluble TL1A induces CD4+ T cell proliferation and cytokine secretion. TL1A bound to DR3 can switch immune response towards type 1 T-helper cells. Dysregulation can lead to pathologies such as inflammatory bowel disease (IBD) and other auto-immune diseases. TL1A is thus an attractive target for auto-immune disease treatment.
Storage/Stability
At least 6 months at -80°C.
Assay Conditions
Dose response of Anti-TL1A antibody. TL1A-Responsive Luciferase Reporter Jurkat cells (BPS Bioscience #78811) were plated according to the cell line protocol. A serial dilution of anti-TL1A antibody was prepared and pre-incubated with 1 μg/ml human TL1A for 1 hour. After the 1-hour incubation, the anti-TL1A antibody and TL1A mixture was added to the cells. The plate was incubated at 37°C with 5% CO2 for 5 hours before being assayed using the One-Step™ Luciferase Detection Reagent (BPS Bioscience #60690). Data is shown as the percent luminescence of NF-kB luciferase reporter expression, in which the absence of antibody is defined as 100%.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for studying the neutralization of TL1A in ELISA and in cellular assays.
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