TL1A-Responsive Luciferase Reporter Jurkat Cell Line
TNF-like ligand 1A (TL1A)-Responsive Luciferase Reporter Jurkat Cell Line is a TL1A-responsive DR3/NF-kB luciferase reporter Jurkat cell line expressing firefly luciferase under the control of an NF-kB response element, and with stable expression of human DR3 (death receptor 3; TNFRSF25; NM_003790.3). Expression of the firefly luciferase reporter is driven by NF-kB response elements located upstream of the minimal TATA promoter. Activation of the NF-kB signaling pathway by the DR3 ligand TL1A (TNF-like Protein 1A; TNFSF15) can be monitored by measuring luciferase activity.
This cell line has been validated to be responsive to TL1A and TNF-α stimulation. TL1A stimulation was blocked by the neutralizing anti-TL1A antibody (#101729) and DcR3 (decoy receptor 3).
Figure 1: Schematic representation of the TL1A response in TL1A Responsive Luciferase Reporter Jurkat Cell Line.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of TL1A:DR3 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
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Materials Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 2 | BPS Bioscience #60184 |
| Growth Medium 2A | BPS Bioscience #60190 |
Materials Required for Cellular Assay
| Name | Ordering Information |
| TL1A, His-Tag, Avi-Tag Recombinant | BPS Bioscience #101880 |
| Anti-TL1A Antibody | BPS Bioscience #101729 |
| Recombinant Human TNF-alpha Protein | R&D Systems #210-TA |
| Human Recombinant DcR3/TNFRSF6B Fc chimera | R&D Systems #142-DC |
| NF-κB Luciferase Reporter Jurkat Cell Line | BPS Bioscience #60651 |
| Growth Medium 2A | BPS Bioscience #60190 |
| Assay Medium: Thaw Medium 2 | BPS Bioscience #60184 |
| 96-well tissue culture treated, white, clear-bottom assay plate | |
| ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
| Luminometer |
The cell line has been screened to confirm the absence of Mycoplasma species
TNF-like ligand 1A (TL1A, also known as Vascular endothelial growth inhibitor, VEGI or TNFSF15) is an anti-angiogenic cytokine. It is an important mediator of inflammation, participating in innate and adaptive immune homeostasis through binding to its receptor, DR3 (death receptor 3), activating downstream signaling. Numerous studies showed that soluble TL1A can be detected in the serum of patients with T-cell mediated autoimmune diseases like rheumatoid arthritis, psoriatic arthritis, and inflammatory bowel disease. In addition, recent clinical studies suggested that anti-TL1A antibody treatment is a promising therapeutic approach in inflammatory and auto-immune disorders.
Xu WD, et al., 2022 Front. Immunol. 13: 891328.
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