TL1A-Responsive Luciferase Reporter Jurkat Cell Line

Catalog #
78811
$11,190 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

TNF-like ligand 1A (TL1A)-Responsive Luciferase Reporter Jurkat Cell Line is a TL1A-responsive DR3/NF-kB luciferase reporter Jurkat cell line expressing firefly luciferase under the control of an NF-kB response element, and with stable expression of human DR3 (death receptor 3; TNFRSF25; NM_003790.3). Expression of the firefly luciferase gene is driven by NF-kB response elements located upstream of the minimal TATA promoter. Activation of the NF-kB signaling pathway by the DR3 ligand TL1A (TNF-like Protein 1A; TNFSF15) can be monitored by measuring luciferase activity. 

This cell line has been validated to be responsive to TL1A and TNF-α stimulation. TL1A stimulation was blocked by the neutralizing anti-TL1A antibody (BPS Bioscience #101729).

Figure 1: Schematic representation of the TL1A response in  TL1A Responsive Luciferase Reporter Jurkat Cell Line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
TL1A responsive cell line, TNFa responsive cell line
Product Info
Storage and Usage
Citations
Host Cell Line
Jurkat cells (clone E6-1), Human T lymphoblast, suspension
Supplied As
Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Materials Required But Not Supplied

Materials Required for Cell Culture

Name Ordering Information
Thaw Medium 2 BPS Bioscience #60184
Growth Medium 2A BPS Bioscience #60190


Materials Required for Cellular Assay

Name Ordering Information
TL1A, His-Tag, Avi-Tag Recombinant BPS Bioscience #101880
Anti-TL1A Antibody BPS Bioscience #101729
Growth Medium 2A BPS Bioscience #60190
Assay Medium: Thaw Medium 2 BPS Bioscience #60184
96-well tissue culture treated, white, clear-bottom assay plate  
ONE-Step™ Luciferase Assay System BPS Bioscience #60690
Luminometer  
UniProt #
TL1A: O95150; DR3: Q93038
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species

Background

TNF-like ligand 1A (TL1A, also known as Vascular endothelial growth inhibitor, VEGI or TNFSF15) is an anti-angiogenic cytokine. It is an important mediator of inflammation, participates in innate and adaptive immune homeostasis through binding to its receptor, DR3, activating downstream signaling. Numerous studies showed that soluble TL1A can be detected in the serum of patients with T-cell mediated autoimmune diseases like rheumatoid arthritis, psoriatic arthritis, and inflammatory bowel disease. In addition, recent clinical studies suggested that anti-TL1A antibody treatment is a promising therapeutic approach in inflammatory and auto-immune disorders.