The CSF1R Kinase Assay Kit is designed to measure CSF1R kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
Need us to run inhibitor screens or profile your compounds against CSF1R? Check out our Kinase Screening Services.
The assay kit comes in a convenient 96-well format, with enough purified recombinant CSF1R kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Colony Stimulating Factor 1 Receptor (CSF1R, also known as CSFR, CD115, and M-CSF-R) is a tyrosine kinase transmembrane receptor activated either by CSF1 (MCSF) or interleukin-34 (IL-34), causing homodimerization and activation of the kinase activity. CSF1R is expressed on the surface of monocytes and macrophages, controlling their growth, survival, or differentiation. A number of tumor cells overexpress the cytokine CSF1, driving the development and survival of Tumor-Associated Macrophages, which in turn suppress the local immune response to the cancer
Storage/Stability
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Applications
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput screen (HTS) applications.
Shipping Temperature
-80°C
Notes
Troubleshooting Guide Visit Assay-kits-faq for detailed troubleshooting instructions. For further questions, please email [email protected]
Contraindications
The final concentration of DMSO in the assay should not exceed 1%.
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
Powered by Bioz
